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micro biospin6 columns  (Bio-Rad)


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    Structured Review

    Bio-Rad micro biospin6 columns
    Micro Biospin6 Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro+biospin6+columns/Micro+Bio-Spin+6+Columns/pm27563403__ml6b00169_si_001-42-26-29
    Average 96 stars, based on 1537 article reviews
    micro biospin6 columns - by Bioz Stars, 2026-09
    96/100 stars

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    Titration:

    Article Title: Dynamic Long-Range Interactions Influence Substrate Binding and Catalysis by Human Histidine Triad Nucleotide Binding Proteins (HINTs), Key Regulators of Multiple Cellular Processes and Activators of Antiviral ProTides
    Article Snippet: Isothermal titration calorimetry (ITC) experiments were conducted in a MicroCal Auto-ITC200 system (GE Healthcare life sciences). .. All titration experiments were performed at 25 °C in 10 mM Tris, 150 mM NaCl, pH 7.2 (ITC buffer ). hHINT1 and hHINT2 were exchanged into ITC buffer using Micro Biospin6 columns (BioRad, USA) so that all experimental buffer was identical. hHINT protein concentration (5 - 600 μM) depended on its affinity for the ligand and a 10:1 ligand to protein ratio was utilized. ..

    Article Title: Sulfamide and sulfamate inhibitors of hHint1
    Article Snippet: Isothermal Titration Calorimetry (ITC) ITC experiments were conducted on a MicroCal Auto-ITC200 system (GE Healthcare life sciences). .. All titration experiments were performed at 20° C. in ITC buffer (10 mM Tris, 150 mM NaCl, pH 7.5). hHint1 was exchanged into ITC buffer using Micro biospin6 columns (BioRad, USA) and final protein concentrations were determined as described above. ..

    Article Title: Supporting Information Design, Synthesis and Characterization of Sulfamide and Sulfamate Nucelotidomimetic Inhibitors of hHint1
    Article Snippet: ITC experiments were conducted on a MicroCal Auto-ITC200 system (GE Healthcare life sciences). .. All titration experiments were performed at 20 °C in ITC buffer (10 mM Tris, 150 mM NaCl, pH 7.5). hHint1 was exchanged into ITC buffer using Micro biospin6 columns (BioRad, USA) and final protein concentrations were determined as described above. ..

    Article Title: Inhibition of HINT1 Modulates Spinal Nociception and NMDA Evoked Behavior in Mice.
    Article Snippet: The interactions between the mu-opioid (MOR) and N-methyl-D-aspartate receptor (NMDAR) constitute an area of intense investigation due to their contributions to maladaptive neuroplasticity.. Recent evidence suggests that their association requires the involvement of histidine triad nucleotide-binding protein (HINT1) with the enzyme’s active site being critical in its regulatory role.. Since it is known that spinal blockade of NMDA receptors prevents the development of opioid analgesic tolerance, we hypothesized that spinal inhibition of the HINT1 enzyme may similarly inhibit opioid tolerance.

    Protein Concentration:

    Article Title: Dynamic Long-Range Interactions Influence Substrate Binding and Catalysis by Human Histidine Triad Nucleotide Binding Proteins (HINTs), Key Regulators of Multiple Cellular Processes and Activators of Antiviral ProTides
    Article Snippet: Isothermal titration calorimetry (ITC) experiments were conducted in a MicroCal Auto-ITC200 system (GE Healthcare life sciences). .. All titration experiments were performed at 25 °C in 10 mM Tris, 150 mM NaCl, pH 7.2 (ITC buffer ). hHINT1 and hHINT2 were exchanged into ITC buffer using Micro Biospin6 columns (BioRad, USA) so that all experimental buffer was identical. hHINT protein concentration (5 - 600 μM) depended on its affinity for the ligand and a 10:1 ligand to protein ratio was utilized. ..

    Purification:

    Article Title: Repression of gonadotropin-releasing hormone gene expression by androgen receptor
    Article Snippet: One pmol of d.s. oligonucleotide was end-labeled with 0.34 millicuries [ 32 P] using T4 Polynucleotide Kinase (New England Biolabs, Inc., Beverly, MA), then incubated for 1 hour at 37°. .. After the reaction was stopped with 30 μl Tris-EDTA (TE) buffer, the probe was purified using Micro BioSpin6 Columns #732-6221 (Bio-rad laboratories, Inc., Hercules, CA). ..



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